PERBANDINGAN PAPARAN ASAP ROKOK KONVENSIONAL DAN ROKOK HERBAL PADA MENCIT (Mus musculus) TERHADAP PERBANDINGAN GAMBARAN HISTOLOGI PARU

Main Article Content

Nurul Hidayah Olan Rahayu Puji Astuti Mussa Rondius Solfaine Yudha Sapto Utomo

Abstract

The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes.


The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p> 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p <0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p <0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes.

Keywords:
Herbal cigarettes, Conventional cigarettes, Lung histological damage
References
Aji, Amri., Leni, Maulinda., Sayed, Amin. 2015. Isolasi nikotin dari putung rokok sebagai insektisida. Jurnal Teknologi Kimia Unimal 4(1):100-120.

Ardy, Carolina., Iwan Sahrial Hamid., Aditya Yudhana., Widjiati., Muhammad Thohawi Elziyad Purnama., Faisal Fikri. 2020. Pengaruh paparan uap rokok elektrik terhadap gambaran histopatologi organ pulmo tikus putih (Rattus norvegicus). J Med Vet. 3(1):38-44.

Azzahra, Nabila Fatimah., Susianti., Khairun Nisa. 2018. Pengaruh pemberian ekstrak kulit manggis (Garcinia mangostana L.) terhadap kerusakan struktur histologis paru mencit jantan galur BALB/c yang diinduksi asap obat nyamuk bakar. Majority. 7(2):86-94.

Avisha, Azarine Neira., Prawesty Diah Utami. 2018. Pengaruh pemberian ekstrak rimpang temulawak (Curcuma xanthorrhiza Roxb.) terhadap jumlah eritrosit mencit (Mus musculus L.) jantan BALB/c yang diinokulasi plasmodium berghei anka. Hang Tuah Medical Journal. 15(2):192-219.

Febrina, Lizma., Helmi., Laode Rijai. 2016. Profil kadar malondialdehida, glukosa dan kolestrol pada tikus putih yang terpapar asap rokok. J.Trop.Pharm.Chem. 3(4):277-282.

Fitria., R.I.N.K Retno Triandhini., Jubhar.C.Mangimbulude., Ferry.F.Karwur. 2013. Merokok Dan Oksidasi DNA. Sains Medika. Salatiga. 5(2):113-120.

Gan, Quan., Jie Yang., Gonghuan, Yang., Maciej, Goniewicz., Neal L. Benowitz., Stanton A.Glanzt. 2009. Chinese herbal cigarettes are as carcinogenic and addictive asregularcigarettes.Cancer Epidemiol Biomarkers Prev. 18(12)3497–3501.

Guseinov, MD., Bobkova, NV., Svistunov, AA., Tarasov,VV, Bokov, DO., Sergunova, EV., Kovaleva, TYu. 2019.Flavonoids in Passiflora incarnata L.dry extract of Russian origin. Pharmacogn J. 11(5):1143-1147.

Hasanah, Uswatul., Rusny., Mashuri Masri. 2015. Analisis pertumbuhan mencit (Mus musculus L.) ICR dari hasil perkawinan inbreeding dengan pemberian pakan AD1 dan AD2. Prosiding Seminar Nasional Mikrobiologi Kesehatan dan Lingkungan. Fakultas Sains dan teknologi UIN Alauddin Makassar. Makassar. 140-145.

Herdiani, Novera., Endah Budi. P. P. 2018. Gambaran histopatologi paru tikus wistar setelah diberi paparan asap rokok. Medical and Health Science Journal. 2(2):7-14.

Hidayat, Rahmat Nur, Adam M. Ramadhan, Rolan Rusli. 2016. Analisis Kadar Nikotin Rokok Herbal Indonesia.Fakultas Farmasi Universitas Mulawarman, Samarinda. 3:72-74.

Kirana, Ramaniya. 2009. Pengaruh pemberian the hijau (Cammelia sinesis) terhadap kerusakan struktur histologis alveolus paru mencit yang dipapar asap rokok. [Skripsi]. Fakultas Kedokteran. Universitas Sebelas Maret.

Muna, Laili, Nailul. 2017. Teratai (Nymphaea stellata Wild) sebagai agen antidiabetik. INPHARMED Jurnal. 1(1):48-54..
Nisa, Khairun., Liana, Sidharti., Muhammad, Farid Adityo. 2015. Pengaruh Kebiasaan Merokok Terhadap Fungsi Paru Pada Pegawai Pria Di Gedung Rektorat Universitas Lampung. Juke unila. 5(9):38-42.

Nuraini, Lisdiana. 2018. Potensi eugenol sebagai agen proteksi kerusakan struktur paru akibat paparan asap rokok. Jurnal MIPA. 41(2):87-95.

Putra, Arba Indra., Rizki Hanriko., Evi Kurniawaty. 2019. Pengaruh efek paparan asap rokok elektrik dibandingkan paparan asap rokok konvensional terhadap gambaran histopatologi paru mencit jantan (Mus muscullus). Majority. 8(1):90-94.

Rohmadianto, Dobby., Nanik Suhartatik., Yannie Asrie Widanti. 2019. Aktivitas antioksidan the rambut jagung (zea mays L.sacharata) dengan penambahan rosella (hibiscus sabdariffa L) dan variasi lama pengeringan. Jurnal Teknologi dan Industri Pangan. 3(2):113-120.

Rohmani, Afiana. 2018. Rokok elektrik dan rokok konvensional merusak alveolus paru. Prosiding Seminar Nasional Unimus. 1: 27-32.

Saleh, Nini, Jayanti., Moses, Soediro. 2017. Serbuk semanggi sebagai minuman herbal. TEKNOBUGA. 4(1):24-29.
Saraswati, Rieke. 2020. Rokok herbal sama bahayanya dengan rokok biasa, ini buktinya. https://www.google.com/amp/s/www.sehatq.com/artikel/rokok-herbal-sama-bahayanya-dengan-rokok-biasa-ini-buktinya/amp [07 April 2020].

Sartika, Nola,Alfieni., Ida Bagus Oka Winaya., Anak Agung A.M.A., I putu Werdikta J.P. 2018. Perubahan histopatologi paru-paru mencit jantan pascapaparan asap rokok elektrik. Indonesia Medicus Veternus. 7(4):402-412.

Suryadinata, Rivan Virlando., Bambang Wirjatmadi., Merryana Adriani. 2016. Pengaruh perubahan hyperplasia sel goblet selama 28 hari paparan asap rokok dengan pemberian antioksidan superoxide dismutase. The Indonesian Journal of Public Health. 11(1): 60-68.

Susilowati, Titik., Suradi., adi prayitno. 2016. Pengaruh pemberian ekstrak tepung daun kelor (Moringa oleifera) terhadap ros (Kadar MDA) dan gambaran histopatologi jaringan paru pada tikus wistar yang diinhalasi asap rokok. Nutrition and health. 1-19.

Ulfah, Safara Malullana., M.Nur Salim., Nazaruddin., Dwinna Aliza., Ummu Balqis., Siti Aisyah. 2016. Gambaran histopatologis paru anjing local (canis lupus familiaris) yang menderita antrakosis. Jurnal Medika Veterinaria. 20(2):105-108.

Ung, Janet Edrina, I Nyoman Sasputra., Debora, S.Liana. 2018. Pengaruh perbedaan waktu paparan asap rokok kretek non filter terhadap gambaran histopatologi paru mencit (Mus muscullus). Cendana Medical Jurnal. 15(3):362-363.

Unitly, Adrien, Jems,Akiles., Nastiti Kusumorini., Srihadi Agungpriyono., Aryani Sismin Satyaningtijas., Arief Boediono. 2018. Analisis mikroskopis paru-paru tikus jantan (rattus norvegicus) yang terpapar asap rokok. Jurnal Biologi Edukasi. 10(2): 8-11.

Wahyuni, Rosa Dwi. 2017. Aspek imunologi chronic obstructive pulmonary diseases (COPD). MEDIKA TADULAKO. 4(1):59-77.

Wulandari, Ratna., Moch, Agus Krisno B., Lud, Waluyo. 2016. Pengaruh berbagai konsentrasi ekstrak bunga mawar merah (Rosa damascene Mill) terhadap stabilitas warna antosianin agar-agar sebagai sumber belajar biologi. Jurnal Pendidikan Biologi Indonesia. 2(1):48-56.

Yanti, A.R., I. Rachmawati., N.A. Fentami., 2015. Petunjuk Praktikum Farmakologi. Esa Unggul. Jakarta.

Article Details

Author Biography

Nurul Hidayah, Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Laboratorium Mikrobiologi Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Most read articles by the same author(s)