VITEK : Bidang Kedokteran Hewan

The Studi Kasus: Multidrug Resistance Terhadap Bakteri Escherichia coli pada Ayam Broiler di Pasar Tradisonal Balongsari, Surabaya

MARIANA FEBRILIANTI PUTRI — 2024-05-28

Escherichia coli is one of the causes of colibacillosis in poultry. Escherichia coli resistant can be transmitted to humans through animal products and animal waste in the environment as a problem in humans and animals. Antibiotics can be continues to experience resistance which will lead to multidrug-resistant (MDR). The aim of this research was to detect multidrug resistance against Escherichia coli bacteria in broiler chickens at the Balongsari traditional market, Surabaya. The samples used were 24 cloacal swabs from broiler chickens. Isolated and identified samples were culture media, in the form of eosin methylene blue agar (EMBA), Gram staining, Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), indole test, methyl Voges Proskauer (MR-VP). Detection of multidrug resistance confirmation with Mueller-Hinton Agar (MHA) media. The results of isolation and identification of Escherichia coli bacteria in this study showed 83%. Antibiotic resistance testing using the diffusion method showed that the highest antibiotic resistance results occurred in the antibiotics ciprofloxacin 85%, tetracycline 60%, kanamycin 45%, chloramphenicol 25% and aztreonam 20%. The multidrug resistance results showed that Escherichia coli bacteria was 45%. This data confirms the existence of Multidrug resistance against Escherichia coli bacteria in broiler chickens at the Balong Sari traditional market in Surabaya which has an impact on public health.

THE EFFECT OF Averrhoa bilimbi EXTRACT ON THE DUODENUM HISTOPATHOLOGY IN RATS INFECTED WITH Escherichia coli

Aldin Akbar Rahmatullah — 2024-05-28

This study aimed to prove the efficacy of Averrhoa bilimbi extract on histopathological changes in the duodenum of rats infected with Escherichia coli. It was a laboratory experimental study involving 30 male white rats aged 3 months, weight 250 g divided into six treatment groups: P0 (no E. coli induction + no Averrhoa bilimbi extract), P1 (E. coli induction + no Averrhoa bilimbi extract), and E. coli induction + various concentrations of Averrhoa bilimbi extract (20% in P2, 30% in P3, 50% in P4, and 60% in P5). All treatments lasted for 14 days. On the 15th day, necropsy was performed to collect duodenal tissues, which were then histologically examined using Hematoxylin-Eosin staining. Microscopic observations focused on detecting necrosis, inflammatory cell infiltration, and hemorrhage in the duodenal villi. Data were analyzed using the Kruskall-Wallis test, revealing significant differences between treatments (P < 0.05). The result show that the Averrhoa bilimbi extract did not demonstrate preventive effects against duodenal villi damage, including necrosis, inflammatory cell infiltration, and hemorrhage. In conclusion, the administration of Averrhoa bilimbi extract to rats infected with Escherichia coli did not show effectiveness in preserving the histopathological integrity of the duodenal small intestine in white rats.

A Article Study: Residue Study and Residue Detection of Norfloxacin and Tilosin Antibiotics in Broiler Chickens

Cahyo - Wibisono — 2024-05-28

Antibiotics are drugs that are often used in the chicken farming industry, especially broiler chickens, to treat bacterial infections and secondary infections caused by bacteria. Bacteria are one of the microorganisms that cause digestive and respiratory tract diseases in broiler chickens. The use of antibiotics is increasing every year in relation to treating bacterial infections. The combination antibiotic norfloxacin and tylosin which has a synergistic effect in the antibacterial spectrum and is complementary in the mechanism of action of the drug is often used to treat broiler chickens infected by Gram-positive and Gram-negative bacteria. Norfloxacin is a florquinolone antibiotic which has a broad spectrum, while tylosin is a macrolide antibiotic which also has a broad spectrum. combination of norfloxacin and tylosin in commercial poultry farming in Indonesia. This research aims to find out how many cases of residue and detection of norfloxacin and tylosin residues as well as knowing the levels of norfloxacin and tylosin residues in broiler chickens that exceed the Maximum Residue Limit (BMR) referring to SNI 2000. and also aims to increase awareness of broiler chicken breeders and consumers about effects of norfloxacin and tylosin residues on broiler chicken products. Norfloxacin and tylosin residues in broiler chickens were found in several literatures, both in cases of residues and residue detection. From 2010 to 2023, several studies even found no cases of residues of the antibiotics norfloxacin and tylosin in broiler chickens.

Chronic Kidney Disease in Female Domestic Short Hair Cat

Ifara Umaiyah — 2024-05-28

Chronic kidney disease (CKD) is a decrease or loss of the kidney's ability to excrete unused products, concentrate urine and convert electrolytes. A female Domestic short hair cat with clinical findings of Polyuria/polydipsia, decreased muscle mass, anorexia, dehydration, hypersalivation. Defecation was found a solid, dry, black-brownish yellow consistency (Score 2) and urine was dark yellow. The physical examination revealed a drastic decrease in body temperature and tachycardia. Hematological examination showed leukocytosis and hypochromic macrocytic anaemia. The result of blood smear tests showed the presence of neutrophils and other lymphocytes in large numbers. Blood chemistry analysis showed an increase BUN and creatinine (Azotemia) and also increase SGOT. Urinalysis examination revealed proteinuria, ketonuria, urobilinogenuria and hypersthenuria. Fecal examination revealed positive helminthiasis in the form of Toxocara cati eggs which was confirmed by the finding of adult worms in the duodenum during necropsy. The therapy given to the case cat included 50 ml/kgBW Ringer's lactate, 0,5 ml/kgBW Amoxicillin-Clavulanate, 2 mg/kgBW Ranitidine, 0,2 mg/kgBW Ipakitin® and 0,5 ml/kgBW Imboost®. A week after therapy, the cat's condition deteriorated further, with an undetectable temperature, seizures, critical condition and not being able to survive, so a necropsy was carried out. Macroscopic results of the kidneys during the necropsy procedure showed that the korteks of kidneys were pale with no change in size. The histophatology finding in the kidneys are necrosis and fibrosis tubulointerstitial. Microscopy finding in duodenum is inflammation and adult worm Toxocara cati in the lumen duodenum. The conclusion is this cat with diagnose Chronic kidney disease with histophatologycal finding is glomerulonephritis and duodenitis.

THE EFFECT OF GREEN TEA (Camellia sinensis) EXTRACT ON THE THICKNESS OF BRONCHIAL EPITHELIUM AND PULMONARY ARTERY WALL OF MICE (Mus musculus) EXPOSED TO CIGARETTE SMOKE

Aldin Akbar Rahmatullah — 2024-05-28

The study aims to investigate the impact of green tea extract on the thickness of bronchial epithelium and pulmonary artery walls in mice lungs exposed to cigarette smoke. Twenty-five 12-week-old mice (Mus musculus) weighing between 20-25 grams were used in the experiment. The control group (K(-)) received no cigarette smoke exposure and was administered 0.5 mL of 1% Na-CMC, while the positive control group (K(+)) was exposed to cigarette smoke without green tea leaf extract and received 0.5 ml of 1% Na-CMC. Groups exposed to cigarette smoke (P1, P2, and P3) were given green tea extract at doses of 20 mg/kgBW, 40 mg/kgBW, and 60 mg/kgBW respectively, each in a daily dose of 0.5 ml. Mice were exposed to one cigarette per group per day. The findings indicate that administering green tea extract has the potential to reduce the thickening of both the bronchial epithelium and pulmonary artery walls, with the optimal dose being 40 mg/kgBW. Analysis using ANOVA revealed significant differences in the thickness of the bronchial epithelium and pulmonary artery walls among the groups (p < 0.05). This study demonstrates that administering green tea extract suspension (Camellia sinensis) can effectively mitigate the thickening of the bronchial epithelium and pulmonary artery walls in mice lungs exposed to cigarette smoke.

Studi Kasus Identifikasi Infeksi Strongylus dan Coccidia Pada Sapi Perah Peranakan Friessian Holstein di Unit Pelaksana Teknis Pembibitan Ternak dan Hijauan Makanan Ternak Batu

Ganang Rilo Pambudi — 2024-05-28

Penelitian ini dilaksanakan untuk mengetahui risiko infeksi endoparasit Strongyloides dan Coccidia di Unit Pelaksana Teknis Pembibitan Ternak dan Hijauan Makanan Ternak Batu. Sebanyak 62 sampel feses sapi perah peranakan frissiean holstein diperiksa dengan menggunakan metode apung dan sedimentasi. Pemeriksaan dilaksanakan dengan mengambil feses segar yang dikoleksi dan diperiksa di Unit Pelaksana Teknis Laboratorium Kesehatan Hewan Malang. Hasil pemeriksaan menunjukkan adanya 11 ekor (17,7%) terinfeksi Strongyloides dan 4 ekor (6,5%) terinfeksi Coccidia. Kebersihan kandang dan program pencegahan endoparasit menjadi faktor utama dalam eradikasi endoparasit di Unit Pelaksana Teknis Pembibitan Ternak dan Hijauan Makanan Ternak Batu.

Kata kunci: coccidia, endoparasit, prevalensi, strongyloides.

The EFEKTIVITAS EKSTRAK ETANOL DAUN BANDOTAN (Ageratum conyzoides) TERHADAP Salmonella enterica serovar typhimurium SECARA IN VITRO

daniel daniel daniel — 2024-05-28

Bandotan leaves (Ageratum conyzoides) are one of the traditional medicinal plants that are trusted empirically by the community to treat various diseases. Several studies report the potential of bandotan leaves as an antibacterial because they contain the chemical compounds trepanoids, flavonoids, alkaloids, essential oils, and tannins. This study aims to determine the effectiveness of bandotan leaves on the growth of Salmonella enterica serovar typhimurium bacteria in vitro. This research used the Kirby Bauer disk diffusion method with the antibiotic chloramphenicol as a positive control, CMC 1% as a negative control, and an ethanol extract of bandotan leaves with concentrations of 25%, 50%, and 75%. Re-identification of bacteria was carried out using Gram staining, and effectiveness testing was carried out on Mueller Hinton Agar (MHA) media. The results of the study on Gram staining showed uniform morphological characteristics of Salmonella enterica serovar Typhimurium bacteria and the diameter of the inhibition zone formed at concentrations of bandotan leaf ethanol extract of 25% (8.02 mm ± 0.41), 50% (8.37mm ± 0.69) and 75% (8.78mm ± 0.78). It can be concluded that the ethanol extract of bandotan leaves at concentrations of 25%, 50%, and 75% has a weak category of inhibitory activity against the growth of Salmonella enterica serovar Typhimurium bacteria in vitro.