VITEK : Bidang Kedokteran Hewan https://vitek-fkh.uwks.ac.id/index.php/jv <div class="page-header-hm"> <h4>Vitek : Bidang Kedokteran Hewan</h4> </div> <div class="media-body"> <div class="thumbnail-hm col-md-4 col-sm-4 col-xs-12"> <p align="center"><img class="img-responsive" src="/public/site/images/andisetyaji/cover1.png"></p> <p style="color: #999; font-weight: bold; font-size: 12px;" align="center">e-ISSN : <a href="http://u.lipi.go.id/1550672504" target="blank">2685-8894</a><br>p-ISSN : <a href="http://issn.lipi.go.id/issn.cgi?daftar&amp;1438660136&amp;1&amp;&amp;" target="blank">2460-9773</a></p> </div> <div class="col-md-8 col-sm-8 col-xs-12"> <div> <p><a href="#">Support Open Acces</a></p> <h4>Co-Editors-in-Chief:</h4> <p><a href="https://vitek-fkh.uwks.ac.id/index.php/jv/about/editorialTeam">View Editorial Team</a></p> <hr> <p><strong>Jurnal Vitek Bidang Kedokteran Hewan</strong> terbit 1 kali dalam 1 tahun.<br>Merupakan media yang memuat hasil penelitian, kajian ilmiah, laporan kasus lapangan dan juga sarana komunikasi bagi Peneliti, Pemerhati dan Pengelola Penelitian Kesehatan Hewan</p> <hr> <p><strong>Diterbitkan dan dikelola oleh : </strong><br>Fakultas Kedokteran Hewan <br>Universitas Wijaya Kusuma Surabaya</p> <hr> <p>SK no. 0005.26858894/JI.3.1/SK.ISSN/2019.</p> </div> </div> </div> en-US jurnalvitek@gmail.com (Intan Permatasari Hermawan, drh.,M.Si) vitek@uwks.ac.id (Nurul) Tue, 10 Nov 2020 00:00:00 +0700 OJS 3.1.0.1 http://blogs.law.harvard.edu/tech/rss 60 KAJIAN ARTIKEL: POTENSI PLASMA NON TERMAL SEBAGAI KANDIDAT TERAPI MASTITIS SUBKLINIS https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/41 <p><span class="fontstyle0">Subclinical mastitis is the occurrence of inflammation in the parenchymal tissue of mammary<br>glands due to bacterial contamination that does not show clinical symptoms lead to impacted on<br>economic losses. The highest incidence of subclinical mastitis is the presence of Staphylococcus<br>aureus bacteria contamination. Subclinical mastitis in Indonesia is reported to reduce milk production<br>by up to 53.5%. Pathogenesis of Staphylococcus aureus occurs in the presence of biofilm formation as<br>bacterial immune system to protecting itself from disinfectants and antibiotics. In the last few decades,<br>the development of plasma technology has been widely used in the medical field as cancer therapy,<br>reducing pain, accelerating wound healing and tissue sterilization. There is no study that using the<br>concept of utilizing Non Termal Plasma in the field of veterinary science, especially in mastitis<br>therapy. Non Termal Plasma is able to decontaminate Staphylococcus aureus through inhibition and<br>destruction of biofilms. The mechanism of action of Non Termal Plasma in repairing mammary gland<br>tissue is through the destruction of DNA in the Reactive Oxygen Species (ROS) process by increasing<br>the growth factor TGF-</span><span class="fontstyle2"></span><span class="fontstyle0">1 which accelerates the process normal cell regeneration. The objective of<br>this study is to reviewing the efficacy of plasma non termal as subclinical mastitis theraphy. This study<br>was conducted through a narrative review approach by collecting international and national<br>literature with interval time of publication within 2010 – 2020.</span></p> Elfahra Casanza Amalda, Farah Alhamidah, Yudit Oktanella, Muhammad Khuzain ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/41 Tue, 10 Nov 2020 00:00:00 +0700 EFEKTIVITAS PENGASAPAN MENGGUNAKAN KAYU KESAMBI (Schleichera oleosa) DIBANDINGKAN DENGAN BATOK KELAPA (Cocos nucifera) PADA DAGING BABI TERHADAP TPC, ORGANOLEPTIC DAN AWAL PEMBUSUKAN https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/46 <p><span class="fontstyle0">The study was conducted to determine the effect of fogging using kesambi wood<br>(Schleichera Oleosa) compared to coconut shells (Cocos Nucifera) in pork on TPC, organoleptics<br>and early decay. The experimental design used was a completely randomized design with four<br>treatments with a total of six replications. The treatments applied were P1 fogging for 30 minutes<br>using wood kesambi (Scheleichera Oleosa), P2 fogging for 30 minutes using coconut shells (Cocos<br>Nicifera), P3 fogging for 45 minutes using kesambi wood (Scheleichera Oleosa), P4 fogging for 45<br>minutes using coconut shells (Cocos Nicifera), P3 fogging for 45 minutes using wood cage<br>(Scheleichera Oleosa), P4 fogging for 45 minutes using coconut shells (Cocos Nicifera), P3 fogging<br>for 45 minutes using wood cage (Scheleichera Oleosa), P4 fogging for 45 minutes using coconut<br>shells (Cocos Nicifera), P3 fogging for 45 minutes using wood cage (Scheleichera Oleosa) coconut<br>(Cocos Nucifera). The results of the analysis of total plate count (TPC) showed that there was no<br>real difference. The results of the color and taste organleptic analysis showed that there was no<br>significant difference between P1, P2, P3 and P4 where P ˃ 0.05, while the texture and aroma test<br>showed significant changes between P1, P2, P3 and P4 where P ˂ 0.05. In the initial decay test<br>showed that p1 had the lowest positive results of the initial decay test that is 1 positive sample from<br>a total of 6 samples.</span></p> Sheila Marty Yanestra, Junianto Wika Adi Pratama, Asih Rahayu, Patrianus Tomy ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/46 Tue, 10 Nov 2020 00:00:00 +0700 PERBANDINGAN KADAR LEMAK DAN BERAT JENIS SUSU SAPI PERAH Friesian Holstein (FH) DI BENDUL MERISI, SURABAYA (Dataran Rendah) dan NONGKOJAJAR, PASURUAN (Dataran Tinggi) https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/47 <p><em>This study aims to compare the quality of&nbsp; Friesian Holstein (FH) dairy cows in Bendul Merisi, Surabaya (Lowlands) and Nongkojajar, Pasuruan (Highlands), with the physical quality standard of fresh milk which is the national reference or SNI. This study used 60 samples of cow's milk, 30 samples from Bendul Merisi, and 30 samples from Nongkojajar. The sample was tested in the laboratory using the fat content and specific gravity test, the data obtained were calculated using the T-test formula. The data obtained from the average fat content test in Nongkojajar was 3.3685%, and the average fat content in Bendul Meisi was 4.8003%. The average density at Nongkojajar is 1.024767 mg / dl, the average density at Bendul Merisi is 1.28067 mg / dl. Based on the results of statistical calculations resulted in a significant difference (P &lt;0.01) in the fat content test, statistical calculations resulted in a significant difference (P &lt;0.01) in the specific gravity test.</em></p> Ruswandono Wirjatmadja, Olan Rahayu Puji Astuti Mussa, Ratna Widyawati, M Dzaki Wiranda Pratama ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/47 Tue, 10 Nov 2020 00:00:00 +0700 GAMBARAN HISTOPATOLOGI PARU PADA MENCIT (Mus musculus) YANG DIPAPAR ASAP ROKOK KRETEK AKIBAT PENGARUH PEMBERIAN INFUSA TEH HIJAU (Camellia sinensis) https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/53 <p><em>This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung or</em></p> <p><em>This study was conducted to determine the effect of antioxidant administration from green tea infusion (Camellia sinensis) on damage to the histological structure of the lung exposed to clove cigarette smoke in mice (Mus muscullus). Free radicals in cigarette smoke such as tar, nicotine, and carbon monoxide can cause various damages to the lung organs due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%.</em></p> <p><em>gans due to oxidative stress caused by the reaction of ultrafine particles with cells. A total of 27 mice were taken from the Biochemical Laboratory of Airlangga University, Surabaya. In this study mice (Mus muscullus) were exposed to kretek cigarette smoke in the smoking chamber for 15 minutes in a period of 2 weeks, used 2 variants of antioxidant doses of green tea infusion of 0.45 gr to 0.6 gr. After the treatment was completed, the preparation of the mice's pulmonary organ preparations and damage to the structure of the lung organ were observed in 9 visual fields. The results showed that there was a relationship between the amount of cigarette smoke exposed and damage to the lung organs of mice when given antioxidant green tea. The administration of green tea antioxidants reduced inflammation of the lung organ infiltration of inflammatory cells by 44%, edema by 55%, and destruction of septa alveoli by 88%.</em></p> Dyah Widhowati, Rondius Solfaine, Olan Rahayu Puji Astuti Mussa, Apriska Ayuningtias ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/53 Tue, 10 Nov 2020 00:00:00 +0700 PERBANDINGAN PAPARAN ASAP ROKOK KONVENSIONAL DAN ROKOK HERBAL PADA MENCIT (Mus musculus) TERHADAP PERBANDINGAN GAMBARAN HISTOLOGI PARU https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/55 <p><em>The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p&gt; 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p &lt;0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p &lt;0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes.</em></p> <p><em>The purpose of this study was to determine the comparison of conventional cigarette smoke and herbal cigarettes against histological damage in the lungs of mice. The research method uses a completely randomized design with 21 mice aged 8-12 weeks and divided into 3 treatment groups. Group P0 was not given exposure to cigarette smoke, group P1 was exposed to conventional cigarette smoke tar content was 39 mg nicotine 2 mg, group P2 was exposed to herbal cigarette smoke tar content was 41.60 mg nicotine 1.64 mg. On the 15th day the mice were deuthanated for pulmonary extraction and haematoxyline eosin staining was made. Data analysis using one way ANOVA. The results of this study obtained scoring lung damage to edema lesions in P0, P1, P2 Sig 0.246 (p&gt; 0.05). Inflammation of inflammatory cells at P0, P1, P2 Sig 0.000 (p &lt;0.05). Destruction of alveolar septum at P0, P1, P2 Sig 0.000 (p &lt;0.05). The conclusion of this study is the difference in lung damage in mice in the form of edema, infiltration of inflammatory cells, destruction of the alveolar septum between exposure to conventional cigarette smoke and herbal cigarettes.</em></p> Nurul Hidayah (Journal editor); Olan Rahayu Puji Astuti Mussa, Rondius Solfaine, Yudha Sapto Utomo ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/55 Tue, 10 Nov 2020 00:00:00 +0700 OTITIS EKSTERNA AKIBAT INFESTASI Otodectes cynotis PADA KUCING DOMESTIK LONG HAIR https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/58 <p><em>A</em> <em>two</em><em> year</em><em>s</em><em> old male</em><em> cat</em><em> domestic long hair was brought to the Tabby Pet Care clinic with the owner's complaint that the cat often scratches his right and left ears. The results of clinical examination showed scratching marks on the pinna, blackish yellow, wet</em><em>, </em><em>and smelly ear</em><em> serumen</em><em> on the ear canal. Investigations include microscopic examination of ear swabs and cerumen swab cytology. Ear swab examination results observed the presence of Otodectes cynotis and Otodectes cynotis eggs. The results of cytological examination of cerumen swabs using the rapid stain method were observed to have bacterial infections in the form of bacilli, cocc</em><em>us</em><em>, and the presence of neutrophi</em><em>l</em><em> infiltration. Based on clinical symptoms and the results of investigations, the patient was diagnosed with external otitis with a fausta prognosis. Treatment of otitis externa is carried out with topical therapy</em> <em>Ilium® ear drop. Therapy is carried out for seven days. On the eighth day the patient was declared cured and allowed to go home.</em></p> Erfan Andrianto Aritonang, Nindya Kusumawati, Ady Kurnianto ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/58 Tue, 10 Nov 2020 00:00:00 +0700 POTENSI DAUN ASHITABA (Angelica keiskei) SEBAGAI SUMBER FITOBIOTIK DALAM PAKAN TERHADAP PRODUKSI LEMAK ABDOMINAL AYAM BROILER https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/60 <p><em>Ashitaba leaves have many vitamins, minerals, amino acids, antioxidants, and has the potential to be a feed supplement to chicken growth. The purpose of this study was to determine the potential of Ashitaba leaf flour as a source of probiotics in feed on levels of broiler abdominal fat production. 25 DOC broilers were kept for 35 days. The treatment was the addition of Ashitaba leaf flour in basal feed with different levels ; P0 (0% Ashitaba Leaf Flour + 98% Basal Feed + 2% Filler); P1 (0.5% Ashitaba Leaf Flour + 98% Basal Feed + 1.5% Filler); P2 (1% Ashitaba Leaf Flour + 98% Basal Feed + 1% Filler); P3 (1.5% Ashitaba leaf meal + 98% Basal feed + 0.5% Filler) and; P4 (2% Ashitaba leaf flour + 98% Basal feed + 0% Filler). The data obtained were analyzed using variance and if there were significant differences it would be further tested with the Duncan Distance Test. The results showed that giving Ashitaba Leaf Flour up to a level of 2% had a significant on increasing the weight of broiler (P&gt; 0.05) but had no significant (P&gt; 0.05) on the weight of abdominal fat and the percentage of abdominal fat.</em></p> Dina Oktaviana Spt, Mashur Mashur, Candra Dwi Atma, Maratun Janah, Kunti Tirta Sari ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/60 Tue, 10 Nov 2020 00:00:00 +0700 PEMANFAATAN EKSTRAK BUNGA KECOMBRANG (Etlingera elatior jack R.M.sm) SEBAGAI LARVASIDA NYAMUK (Culex quinquifasciatus Say) https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/45 <p><em>This study aims to determine the effectiveness of kecombrang flower extract (Etlingera elatior jack R.M.sm) on the death of Culex quenquefasciatus Say larvae. Experimental animals used were Culex quenquefasciatus Say larvae, amounting to 625 tails. The design used was a randomized complete design with 5 treatments and 5 replications. The five treatments consisted of two test groups, namely the treatment group consisting of concentrations of 5%, 10%, 15%, and the control group consisting of positive control namely abate and negative control namely aquades. Observation of mosquito larvae death was done every 2 hours for 12 hours. The data obtained were analyzed using the one-way ANOVA test and continued with the Duncan test</em><em>.</em><em> Based on the results of the one way ANOVA calculation the value of sig.0.00 &lt;0.05 means that there are significant differences between treatments. Duncan test results on the treatment of 10% and 15% kecombrang flower extract have the ability as a larvicide high and equal to positive control (abate). Based on the results of the study it was concluded that the extract of kecombrang flower (Etlingera elatior jack R.M.sm) had the ability to larvae against the Culex quenquefasciatus Say mosquito.</em></p> Indra Rahmawati, Bagus Uda Palgunadi, Lailia Dwi Kusuma Wardhani, Ni Made Dewi Lestari ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/45 Tue, 10 Nov 2020 00:00:00 +0700 PEMANFAATAN INFUSA DAUN SIRIH MERAH (Piper crocatum) SEBAGAI ANTI-OBESITAS PADA MENCIT (Mus musculus) https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/49 <p><em>The purpose of this research is to know the effectiveness of red betel leaf infusion (Piper crocatum) as anti-obesity in mice (Mus musculus). Other parameters of this study to know the relationship between changes in blood glucose levels to obesity in experimental animals. Experimental animals used were 24 male mice. The design of this research is completely randomized design (CRD) with 4 treatments 6 </em><em>repetitions. There are four treatmens P0 (Control), P1 (6 mice by giving a betel leaf infusion with a concentration of 5%), P2 (6 mice by giving a betel leaf infusion with a concentration of 10%), and P3 (6 mice by giving a betel leaf infusion with a concentration of red 15%). The treatment is carried out once every day for 21 days. Before treatment, initial weight was weighed and blood glucose level examination continued on the 7th day, on the 14th day, and on the 21st day. Data obtained from the results of weighing and checking blood sugar levels were further analyzed using the ANOVA test which statistically showed no significant changes (P&gt; 0.05) of body weight and blood glucose levels in mice (Mus musculus) given infusion red betel leaf (Piper crocatum).</em></p> Desty Apritya, Miarsono Sigit, Retina Yunani, Fuji Lestari ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/49 Tue, 10 Nov 2020 00:00:00 +0700 RESIDU ANTIBIOTIK PADA SUSU KAMBING PE YANG MENDERITA MASTITIS SUBKLINIS DI DESA AIKMUAL LOMBOK TENGAH https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/36 <p><em>Subclinical mastitis is a disease that often occurs in Etawa (PE) crossbreed goat farms. Farmers in handling this disease usually use antibiotics, giving antibiotics that are not right or according to the procedure can cause residues. The presence of antibiotic residues in goat's milk can have an impact on human health such as poisoning, allergies, and microbiological disorders. This study aims to determine the incidence rate and find the presence of antibiotic residues in the milk of Etawa Peranakan goat who suffer from subclinical mastitis in Aikmual Village, Central Lombok Regency. Test results from 6 samples of etawa-crossed goat milk (PE) suffering from subclinical mastitis using the Bioassay Screening Test method showed negative results containing antibiotic residues. Negative results in the sample are marked by the absence of dead bacteria on paper discs that have been soaked with milk samples from goats that are positive for subclinical mastitis, this is evident from the absence of inhibitory zones formed. The negative effect of this milk sample is due to several possibilities, namely: The stopping period of the drug has been reached and the antibiotics used are in accordance with&nbsp; the&nbsp; dose. The duration of the presence of antibiotics in milk depends on several factors,&nbsp; including: dose, method of administration and type of antibiotic. In&nbsp; general,&nbsp; it is applied&nbsp; that the withdrawal time of narrow spectrum antibiotics is at least 5 days after treatment, for the type of antibiotics that can form a depot (broad spectrum) has a withdrawal time of 1 3 days </em></p> rodi iskandar ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/36 Tue, 10 Nov 2020 00:00:00 +0700 DETEKSI EKTOPARASIT Argulus sp. PADA BUDIDAYA IKAN KARPER (Cyprinus carpio L) DI UPTD- BALAI PENGEMBANGAN BUDIDAYA IKAN AIR TAWAR AIKMEL KABUPATEN LOMBOK TIMUR https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/56 <p><em>Argulus </em>sp<em>. is a fish louse disease that attacks fish on the outside of the body (Ectoparasites) resulting in wounds and bite marks being a trigger factor for the onset of other diseases (secondary diseases) resulting in losses for fish farmers. This research aims to find out if it detected Argulus ectoparasites sp in carper fish cultivation (Cyprinus carpio,L) in UPTD-BPBIAT Aikmel. This study uses a descriptive study design with observational survey type. Fish sampling is carried out using random sampling in one shot using a net with a total of 10 samples. Ectoparasite examination is carried out by making a direct observation then carried out the examination of the head, fins, tail with Alcohol Swabs 70%, . The research results shows that 9 fish were positive infectedwith Argulus </em>sp</p> kunti tirtasari ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/56 Tue, 10 Nov 2020 00:00:00 +0700 PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus Lam.) TERHADAP DEGENERASI SEL GINJAL MENCIT (Mus musculus) YANG DIPAPAR PLUMBUM https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/57 <p><em>Plumbum (Pb) pollution in environment can cause dangerous for animal. Plumbum enter the body through oral cavity and was excreted by kidney and can effect to the kidney function. Defect in kidney can make distraction of metabolism. An effort to prevent deffect in kidney that cause by plumbum can be used antioxidant from Red Fruit plant (Padanus conoideus Lam). The aim of this research was to know the effect of Red Fruit plant with the degrees of kidney cell degeneration that was exposed by plumbum. Type of this research was experimental research that use complete random design with 4 treatment, P0 (aquadest 0,5 ml), P1 (Pb 0,01 mg), P2 (was given with Red Fruit Oil 0,3 ml and Pb 0,01 mg), P3 (was given with Red Fruit Oil 0,8 ml and Pb0,01 mg). The data was analyzed by Kruskal Wallis using SPSS and followed with Mann-Whitney test. The result of this research show that there were significant difference between P0 and P1, P0 and P2, P0 and P3, P1 and P3 (P&lt;0,05), but did not showed significant difference (p&gt;0.05) with P2 and P3. Base on this research can be concluded that giving red fruit oil can decrease the degrees of cell degeneration in mouse kidney that was exposed by plumbum. </em></p> novarina sullsia, Siska Aprilianti, Maratun Janah, Candra Dwi Atma ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/57 Tue, 10 Nov 2020 00:00:00 +0700 EKSTRAK BUAH NANAS TERHADAP JUMLAH TOTAL LEUKOSIT DAN NEUTROFIL IKAN LELE (Clarias batrachus) YANG DIINFEKSI DENGAN Aeromonas hydrophila https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/43 <p><em>The present research to determine the Extract of Pineapple (Ananas comosus) on the survival of catfish infected with A. Hydrophila. The main problem of cultivation of catfish (C. batrachus) is a disease caused by the bacterium Aeromonas hydrophila.Catfish (Clarias batrachus) is a freshwater fish that has several advantages, one of which is a high nutritional content. Test animals used were catfish measuring 15-20cm with a body weight of ± 250gr. Challenge test by injecting A. hydrophila suspension with a dose of 10<sup>8</sup>sel / mm3 as much as 0.1mm intramuscularly. Observations were made for 7 days after infection&nbsp; which included the total number of leukocytes and neutrophils. This study was divided into 5 treatment groups with 5 replications, namely P1 (positive control), P2 (0.1%), P3 (0.3%), P4 (0.5%),&nbsp; P5&nbsp; (0.7%)&nbsp; and soaking Pineapple Fruit Extract for 24 hours. Data analysis in this study used one way ANOVA statistical test. The results showed symptoms of catfish attacked by Aeromonas hydrophila including increased total number of leukocytes and neutrophils and ulceration in catfish. Soaking effect Pineapple fruit extract 0.5% effective dose can reduce the total number of leukocytes and 0.7% effective dose can reduce the total number of neutrophils.</em></p> Ratna Widyawati, Dyah Widhowati, Dhia Nadhifa ##submission.copyrightStatement## https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/43 Tue, 10 Nov 2020 00:00:00 +0700