Profile Protein "Brucella Abortus" Local Isolate with SDS Page Method
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Published
May 18, 2018
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1.
Apritya D, Handijatno D, Ratnasari R. Profile Protein "Brucella Abortus" Local Isolate with SDS Page Method. VITEK : Bidang Kedokteran Hewan [Internet]. 18May2018 [cited 20Apr.2024];4. Available from: https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/3
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Abstract
The aim of this research was to find out the profile protein Brucella abortus local isolate. The samples bacteries were obtained from Balai Besar Veteriner Maros, South Sulawesi.
Whole protein was obtained from Brucella abortus local isolate by ultrasonic homogenizer. The protein profile was analyzed by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) with 4% stacking gel, 12% separating gel and standard of molecular weight was 10 – 200 kDa.
The results of this research showed that the profile protein had seven fractions there were 112 kDa, 72 kDa, 54 kDa, 46 kDa, 41 kDa, 31 kDa and 20 kDa.
Keywords:
Protein, Brucella abortus, local isolate, SDS - PAGE
References
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Meles, W. 2007. Penyakit Sapi Perah Disebabkan Faktor Makanan. Laboratorium Kebidanan. Fakultas Kedokteran Hewan Universitas Airlangga
Neta, A.V.C., Mol, J.P.S., Xavier, M.N., Paixao, T.A., Lage, A.P., dan Santos, R.L. 2009. Pathogenesis of Bovine Brucellosis. The Vet. Journal 184: 146–155.
Nicoletti, P. 1980. The Epidemiology of Bovine Brucellosis. Advances in Veterinary Science and Comparative Medicine. Vol : 24, Hal : 69–95.
Quinn, P.J, B.K. Markey, M.E. Carter, W.L. Donnely and F.C. Leonard. 2002. Veterinary Microbiology and Microbial Disease. Blackwell Publishing. Hal 163 – 167.
Siadat, S,D, Salmani,A,S. Aghasadeghi, M,R. 2012. Brucellosis Vaccines: An Overview, Zoonosis, ISBN: 978-953-51-0479-7, InTech, Available from: http://www.intechopen.com/books/zoonosis/brucellosis-vaccines.
Soebronto. 2003. Ilmu Penyakit Ternak I. Gadjah Mada University Press. Yogyakarta. Hal 464, 465, 474, 475, 483, 484
Sulaiman, I. 2006. Bovine Brucellosis (Bakteriologi - Isolasi dan Identifikasi). Dalam Pedoman Diagnosa Laboratorium Brucellosis Sapi – BBVet Wates. Hal : 1 – 11.