THE EFFECT OF GREEN TEA (Camellia sinensis) EXTRACT ON THE THICKNESS OF BRONCHIAL EPITHELIUM AND PULMONARY ARTERY WALL OF MICE (Mus musculus) EXPOSED TO CIGARETTE SMOKE
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Abstract
The study aims to investigate the impact of green tea extract on the thickness of bronchial epithelium and pulmonary artery walls in mice lungs exposed to cigarette smoke. Twenty-five 12-week-old mice (Mus musculus) weighing between 20-25 grams were used in the experiment. The control group (K(-)) received no cigarette smoke exposure and was administered 0.5 mL of 1% Na-CMC, while the positive control group (K(+)) was exposed to cigarette smoke without green tea leaf extract and received 0.5 ml of 1% Na-CMC. Groups exposed to cigarette smoke (P1, P2, and P3) were given green tea extract at doses of 20 mg/kgBW, 40 mg/kgBW, and 60 mg/kgBW respectively, each in a daily dose of 0.5 ml. Mice were exposed to one cigarette per group per day. The findings indicate that administering green tea extract has the potential to reduce the thickening of both the bronchial epithelium and pulmonary artery walls, with the optimal dose being 40 mg/kgBW. Analysis using ANOVA revealed significant differences in the thickness of the bronchial epithelium and pulmonary artery walls among the groups (p < 0.05). This study demonstrates that administering green tea extract suspension (Camellia sinensis) can effectively mitigate the thickening of the bronchial epithelium and pulmonary artery walls in mice lungs exposed to cigarette smoke.
Alqarni, A. A., Brand, O. J., Pasini, A., Alahmari, M., Alghamdi, A., & Pang, L. 2022. Imbalanced prostanoid release mediates cigarette smoke-induced human pulmonary artery cell proliferation. Respiratory Research, 23(1), 136.
Fu, Y. S., Kang, N., Yu, Y., Mi, Y., Guo, J., Wu, J., & Weng, C. F. 2022. Polyphenols, flavonoids and inflammasomes: the role of cigarette smoke in COPD. European Respiratory Review, 31(164).
Khoirunnisa I, Susilowati S, Maslachah L, Suprayogi TW, Kurnijasanti R, Ratnani H. 2019. The effect of the addition of green tea extract (Camellia sinensis) in egg yolk citrate diluent for quality Sapudi Sheep spermatozoa storage on cold temperature. Ovozoa: J Anim Reprod. 8: 127-31.
Leone, A., & Landini, L. 2013. Vascular pathology from smoking: look at the microcirculation. Current vascular pharmacology, 11(4), 524-530.
Mallah, M. A., Soomro, T., Ali, M., Noreen, S., Khatoon, N., Kafle, A., Feng, F., Wang, W., Naveed, M., & Zhang, Q. 2023. Cigarette smoking and air pollution exposure and their effects on cardiovascular diseases. Frontiers in public health, 11, 967047.
Morales-Mantilla DE, Huang X, Erice P, Porter P, Zhang Y, Figueroa M, Chandra J, King KY, Kheradmand F, Rodríguez A. 2020. Cigarette Smoke Exposure in Mice using a Whole-Body Inhalation System. J Vis Exp. 164: 10.3791/61793.
Sajida, M. V. P., Luqman, E. M., Triana, I. N., & Widjiati, W. 2020. Effect of Green Tea (Camellia Sinensis) Extract Administration on Sperm Quality of Mice (Mus Musculus) Exposed To 2, 3, 7, 8-Tetrachlorodibenzo-PDioxin.International Journal of Pharmaceutical Research (09752366), 12(4).
Suryadinata, R.V. 2018. Pengaruh Radikal Bebas Terhadap Proses Inflamasi pada Penyakit Paru Obstruktif Kronis (PPOK). Amerta Nutrition 2(4): 317-324.
Thorley, A. J. and T. D. Tetley. 2007. Pulmonary Epithelium, Cigarette Smoke, and Chronic Obstructive Pulmonary Disease. International Journal of Chronic Obstructive Pulmonary Disease. 2(4): 409-428.
Wawryk‑Gawda, E., Chylińska‑Wrzos, P., Zarobkiewicz, M. K., Chłapek, K., & Jodłowska‑Jędrych, B. (2020). Lung histomorphological alterations in rats exposed to cigarette smoke and electronic cigarette vapour. Experimental and Therapeutic Medicine, 19(4), 2826-2832.
Zowail, M.E.M., E.H.H. Khater, and M. E. M. Elasrag. 2009. Protective Effect of Green Tea Extract Against Cytotoxicity Induced by Enrofloxacin in Rat Egypt. Acad. Journal Biology. Science. 1 (1): 45-64.