THE EFFECT OF Averrhoa bilimbi EXTRACT ON THE DUODENUM HISTOPATHOLOGY IN RATS INFECTED WITH Escherichia coli
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Published
May 28, 2024
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1.
Rahmatullah A. THE EFFECT OF Averrhoa bilimbi EXTRACT ON THE DUODENUM HISTOPATHOLOGY IN RATS INFECTED WITH Escherichia coli. VITEK : Bidang Kedokteran Hewan [Internet]. 28May2024 [cited 30Oct.2024];14(1):165-72. Available from: https://vitek-fkh.uwks.ac.id/index.php/jv/article/view/285
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Abstract
This study aimed to prove the efficacy of Averrhoa bilimbi extract on histopathological changes in the duodenum of rats infected with Escherichia coli. It was a laboratory experimental study involving 30 male white rats aged 3 months, weight 250 g divided into six treatment groups: P0 (no E. coli induction + no Averrhoa bilimbi extract), P1 (E. coli induction + no Averrhoa bilimbi extract), and E. coli induction + various concentrations of Averrhoa bilimbi extract (20% in P2, 30% in P3, 50% in P4, and 60% in P5). All treatments lasted for 14 days. On the 15th day, necropsy was performed to collect duodenal tissues, which were then histologically examined using Hematoxylin-Eosin staining. Microscopic observations focused on detecting necrosis, inflammatory cell infiltration, and hemorrhage in the duodenal villi. Data were analyzed using the Kruskall-Wallis test, revealing significant differences between treatments (P < 0.05). The result show that the Averrhoa bilimbi extract did not demonstrate preventive effects against duodenal villi damage, including necrosis, inflammatory cell infiltration, and hemorrhage. In conclusion, the administration of Averrhoa bilimbi extract to rats infected with Escherichia coli did not show effectiveness in preserving the histopathological integrity of the duodenal small intestine in white rats.
Keywords:
Averrhoa bilimbi, Duodenum, Escherichia coli, Rattus norvegicus
References
Agnesa, O. S., Susilo, H., & Lestari, S. R. 2017. Aktivitas imunostimulan ekstrak bawang putih tunggal pada mencit yang diinduksi Escherichia coli. Pharmaciana, 7(1), 105.
Aldi,Y. 2016. Uji Efek Imunomodulator Dari Ekstrak Daun Manggis (Garcinia Mangostana L.) Dengan Metode Carbon Clearence Dan Menghitung Jumlah Leukosit Pada Mencit Putih Jantan. Jurnal farmasi. 8(1)
Alhassan, A. M, Ahmed Q. U. 2016. Averrhoa bilimbi Linn.: A Review of its Ethnomedicinal Uses, Phytochemistry, and Pharmacology. J Pharm Bioallied Sci. 8(4):265–71.
Bader, S. M., Preston, S. P., Saliba, K., Lipszyc, A., Grant, Z. L., Mackiewicz, L., Baldi, A., Hempel, A., Clark, M. P., Peiris, T., Clow, W., Bjelic, J., Stutsz, M. D., Arandjelovic, P., Teale, J., Du, F., Coultas, L., Murphy, J. M., Allison, C. C., Pellegrini, M., & Samson, A. L. 2023. Endothelial Caspase-8 prevents fatal necroptotic hemorrhage caused by commensal bacteria. Cell Death & Differentiation, 30(1), 27-36.
Biharee, A., Sharma, A., Kumar, A., & Jaitak, V. 2020. Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance. Fitoterapia, 146, 104720.
Bongers, S. H., Chen, N., van Grinsven, E., van Staveren, S., Hassani, M., Spijkerman, R., Roi, A. T. L. T., Leijte, G. P., Hietbrink, F., Koenderman, L., & Vrisekoop, N. 2021. Kinetics of neutrophil subsets in acute, subacute, and chronic inflammation. Frontiers in immunology, 12, 674079.
D’arcy, M. S. 2019. Cell death: a review of the major forms of apoptosis, necrosis and autophagy. Cell biology international, 43(6), 582-592.
Dutta, S., Sengupta, P., Roychoudhury, S., Chakravarthi, S., Wang, C. W., & Slama, P. 2022. Antioxidant paradox in male infertility:‘A blind eye’on inflammation. Antioxidants, 11(1), 167.
Fahrunida dan Rarastoeti P. 2015. Kandungan Saponin Buah, Daun, dan Tangkai Daun Belimbing Wuluh (Averrhoa blimbi L.). Seminar Nasional Konservasi dan Pemanfaatan Sumber Daya Alam: 220 – 224.
Jiang, L., Zhu, Y., Luan, P., Xu, J., Ru, G., Fu, J. G., Sang, N., Xiong, Y., He, Y., Lin, G., Wang, J., Zhang, J., & Li, R. 2021. Bacteria-anchoring hybrid liposome capable of absorbing multiple toxins for antivirulence therapy of Escherichia coli infection. ACS nano, 15(3), 4173-4185.
Kumar, S., Choubey, A. K., & Srivastava, P. K. 2022. The effects of dietary immunostimulants on the innate immune response of Indian major carp: A review. Fish & Shellfish Immunology, 123, 36-49.
Ledwaba, S. E., Costa, D. V., Bolick, D. T., Giallourou, N., Medeiros, P. H., Swann, J. R., Traore, A. N., Potgieter, N., Nataro, J. P., & Guerrant, R. L. 2020. Enteropathogenic Escherichia coli infection induces diarrhea, intestinal damage, metabolic alterations, and increased intestinal permeability in a murine model. Frontiers in cellular and infection microbiology, 10, 595266.
8
Malech, H. L., DeLeo, F. R., & Quinn, M. T. 2020. The role of neutrophils in the immune system: an overview. Neutrophil: Methods and Protocols, 3-10.
Nassarawa, S. S., Nayik, G. A., Gupta, S. D., Areche, F. O., Jagdale, Y. D., Ansari, M. J., Hemeg, H. A., Al-Farga, A., & Alotaibi, S. S. 2023. Chemical aspects of polyphenol-protein interactions and their antibacterial activity. Critical Reviews in Food Science and Nutrition, 63(28), 9482-9505.
Peng, Z., Wang, X., Huang, J., & Li, B. 2024. Pathogenic Escherichia coli. In Molecular Medical Microbiology (pp. 1065-1096). Academic Press.
Purnamasari, I. W., Astuti P., Ermawati T. 2014. Viabilitas neutrofil yang diinkubasi dalam ekstrak rimpang temulawak (Curcuma Xanthorrhiza) dan dipapar dengan Streptococcus mutans. Jurnal Dentofasial. 2014: 13(3): 135- 406.
Raharjaningtyas, E. R. P. 2013. Pengaruh Pemberian Infusa Daun Sirsak (Annona muricata L) Secara Subkronis Terhadap Gambaran Histopatologis Lambung dan Usus Tikus Putih. [Skripsi]. Fakultas Farmasi Universitas Sanata Dharma Yogyakarta.
Sania, E., Sandy VK., Yohanna A. 2020. Perbandingan Efektivitas Antibakteri Moringa oleifera dan Ziziphus mauritiana dengan Ekstrak Etanol 96% terhadap Escherichia Coli. Sriwijaya Journal of Medicine 3(1):39-46.
Sobrinho, C. Pimentel., J. Lima Godoi, F. Neves Souza ., C. Graco Zeppelini ., V. Espirito Santo ., D. Carvalho Santiago ., R. Sady Alves ., H. Khalil ., T. Carvalho Pereira ., M. Hanzen Pinna., M. Begon ., S. Machado Cordeiro ., J. Neves Reis ., F. Costa . 2020. Prevalence of Diarrheagenic Escherichia coli (DEC) and Salmonella spp. with zoonotic potential in urban rats in Salvador, Brazil .Epidemiology & Infection is published by Cambridge University Press.
Supriyana., Endah A., Sadimin., Wahyu J. D. U. 2019. Kemampuan Obat Kumur Jinten Hitam Sediaan Kantong Celup Terhadap Monosit dan Neutrofil pada Adhesi Streptococcus mutan. Jurnal Link, 15(2):36-41.
Tethool, S. P. 2015. Efek Fraksi Etanol Air Rumput Kebar (Byophitum Petersianum Klotzch) Terhadap Diferensiasi Leukosit Kelinci Hiperlipidemia. Jurnal Ilmu Ternak danTanaman 5 (1).
Wiadnyana, I. M. P., Budiasa, K., & Berata, I. K. 2015. Histopatologi Usus Halus Mencit Pasca Pemberian Ekstrak Etanol Daun Ashitaba Buletin Veteriner Udayana 7(1): 73-79.
Aldi,Y. 2016. Uji Efek Imunomodulator Dari Ekstrak Daun Manggis (Garcinia Mangostana L.) Dengan Metode Carbon Clearence Dan Menghitung Jumlah Leukosit Pada Mencit Putih Jantan. Jurnal farmasi. 8(1)
Alhassan, A. M, Ahmed Q. U. 2016. Averrhoa bilimbi Linn.: A Review of its Ethnomedicinal Uses, Phytochemistry, and Pharmacology. J Pharm Bioallied Sci. 8(4):265–71.
Bader, S. M., Preston, S. P., Saliba, K., Lipszyc, A., Grant, Z. L., Mackiewicz, L., Baldi, A., Hempel, A., Clark, M. P., Peiris, T., Clow, W., Bjelic, J., Stutsz, M. D., Arandjelovic, P., Teale, J., Du, F., Coultas, L., Murphy, J. M., Allison, C. C., Pellegrini, M., & Samson, A. L. 2023. Endothelial Caspase-8 prevents fatal necroptotic hemorrhage caused by commensal bacteria. Cell Death & Differentiation, 30(1), 27-36.
Biharee, A., Sharma, A., Kumar, A., & Jaitak, V. 2020. Antimicrobial flavonoids as a potential substitute for overcoming antimicrobial resistance. Fitoterapia, 146, 104720.
Bongers, S. H., Chen, N., van Grinsven, E., van Staveren, S., Hassani, M., Spijkerman, R., Roi, A. T. L. T., Leijte, G. P., Hietbrink, F., Koenderman, L., & Vrisekoop, N. 2021. Kinetics of neutrophil subsets in acute, subacute, and chronic inflammation. Frontiers in immunology, 12, 674079.
D’arcy, M. S. 2019. Cell death: a review of the major forms of apoptosis, necrosis and autophagy. Cell biology international, 43(6), 582-592.
Dutta, S., Sengupta, P., Roychoudhury, S., Chakravarthi, S., Wang, C. W., & Slama, P. 2022. Antioxidant paradox in male infertility:‘A blind eye’on inflammation. Antioxidants, 11(1), 167.
Fahrunida dan Rarastoeti P. 2015. Kandungan Saponin Buah, Daun, dan Tangkai Daun Belimbing Wuluh (Averrhoa blimbi L.). Seminar Nasional Konservasi dan Pemanfaatan Sumber Daya Alam: 220 – 224.
Jiang, L., Zhu, Y., Luan, P., Xu, J., Ru, G., Fu, J. G., Sang, N., Xiong, Y., He, Y., Lin, G., Wang, J., Zhang, J., & Li, R. 2021. Bacteria-anchoring hybrid liposome capable of absorbing multiple toxins for antivirulence therapy of Escherichia coli infection. ACS nano, 15(3), 4173-4185.
Kumar, S., Choubey, A. K., & Srivastava, P. K. 2022. The effects of dietary immunostimulants on the innate immune response of Indian major carp: A review. Fish & Shellfish Immunology, 123, 36-49.
Ledwaba, S. E., Costa, D. V., Bolick, D. T., Giallourou, N., Medeiros, P. H., Swann, J. R., Traore, A. N., Potgieter, N., Nataro, J. P., & Guerrant, R. L. 2020. Enteropathogenic Escherichia coli infection induces diarrhea, intestinal damage, metabolic alterations, and increased intestinal permeability in a murine model. Frontiers in cellular and infection microbiology, 10, 595266.
8
Malech, H. L., DeLeo, F. R., & Quinn, M. T. 2020. The role of neutrophils in the immune system: an overview. Neutrophil: Methods and Protocols, 3-10.
Nassarawa, S. S., Nayik, G. A., Gupta, S. D., Areche, F. O., Jagdale, Y. D., Ansari, M. J., Hemeg, H. A., Al-Farga, A., & Alotaibi, S. S. 2023. Chemical aspects of polyphenol-protein interactions and their antibacterial activity. Critical Reviews in Food Science and Nutrition, 63(28), 9482-9505.
Peng, Z., Wang, X., Huang, J., & Li, B. 2024. Pathogenic Escherichia coli. In Molecular Medical Microbiology (pp. 1065-1096). Academic Press.
Purnamasari, I. W., Astuti P., Ermawati T. 2014. Viabilitas neutrofil yang diinkubasi dalam ekstrak rimpang temulawak (Curcuma Xanthorrhiza) dan dipapar dengan Streptococcus mutans. Jurnal Dentofasial. 2014: 13(3): 135- 406.
Raharjaningtyas, E. R. P. 2013. Pengaruh Pemberian Infusa Daun Sirsak (Annona muricata L) Secara Subkronis Terhadap Gambaran Histopatologis Lambung dan Usus Tikus Putih. [Skripsi]. Fakultas Farmasi Universitas Sanata Dharma Yogyakarta.
Sania, E., Sandy VK., Yohanna A. 2020. Perbandingan Efektivitas Antibakteri Moringa oleifera dan Ziziphus mauritiana dengan Ekstrak Etanol 96% terhadap Escherichia Coli. Sriwijaya Journal of Medicine 3(1):39-46.
Sobrinho, C. Pimentel., J. Lima Godoi, F. Neves Souza ., C. Graco Zeppelini ., V. Espirito Santo ., D. Carvalho Santiago ., R. Sady Alves ., H. Khalil ., T. Carvalho Pereira ., M. Hanzen Pinna., M. Begon ., S. Machado Cordeiro ., J. Neves Reis ., F. Costa . 2020. Prevalence of Diarrheagenic Escherichia coli (DEC) and Salmonella spp. with zoonotic potential in urban rats in Salvador, Brazil .Epidemiology & Infection is published by Cambridge University Press.
Supriyana., Endah A., Sadimin., Wahyu J. D. U. 2019. Kemampuan Obat Kumur Jinten Hitam Sediaan Kantong Celup Terhadap Monosit dan Neutrofil pada Adhesi Streptococcus mutan. Jurnal Link, 15(2):36-41.
Tethool, S. P. 2015. Efek Fraksi Etanol Air Rumput Kebar (Byophitum Petersianum Klotzch) Terhadap Diferensiasi Leukosit Kelinci Hiperlipidemia. Jurnal Ilmu Ternak danTanaman 5 (1).
Wiadnyana, I. M. P., Budiasa, K., & Berata, I. K. 2015. Histopatologi Usus Halus Mencit Pasca Pemberian Ekstrak Etanol Daun Ashitaba Buletin Veteriner Udayana 7(1): 73-79.