Uji sensitivitas anti bakteri dan anti jamur terhadap megabacterium secara in vitro

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Miranti Candrarisna Edi Boedi Santosa Sudarmanto Indarjulianto Surya Amanu

Abstract

Uji sensitivitas terhadap bakteri megabacterium penyebab Megabacteriosis pada burung import dilakukan dengan menggunakan beberapa anti bakteri seperti : Ampisillin, Enrofloksasin, Eritromisin, Klindamisin, Kloramfenikol, Streptomisin, dan Tetrasiklin. Sedangkan anti jamur yang digunakan : Griseofulvin, Ketokonazol dan Nystatin. Penelitian ini bertujuan mengkaji potensi obat anti bakterial dan anti jamur terhadap megabacterium. Penelitian dilakukan di Laboratorium Mikrobiologi dan Virologi Fakultas Kedokteran Hewan Universitas Gadjah Mada. Pengujian sensitivitas beberapa obat anti bakterial dan anti jamur dilakukan dengan menggunakan agar disc diffusion method (metode penghambatan cakram dish pada media Muller Hilton Agar plat) secara in vitro. Pengamatan dilakukan pada suhu 370C selama 24-48 jam dalam suasana anaerob. Hasil pengamatan berupa pembentukan diameter zona hambatan di sekitar kertas cakram. Hasil penelitian yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan: megabacterium resistensi terhadap Ampisillin, dan megabacterium sangat sensitif terhadap Griseofulvin, kemudian diikuti Enrofloksasin, Klindamisin, Kloramfenikol, Nystatin, Ketokonazol, Eritromisin, Tetrasiklin dan Streptomisin.

Keywords:
antibakterial, anti jamur, zona hambatan, megabacterium
References
Adam, H.R. 1995. Veterinary and Pharmacology and Therapeutics. Edited by H.R. Adam.7th Edition. Lowa State University Press, Lowa 50014.

Amabile, C.F. 1996. Medical Intelligence Unit Antibiotic Resistance From Molecular Basics to Therapeutic Option. R.G. Landes Company Austin, Texas, U.S.A. Copyright 1996 Springer-Verlag, Heidelberg, Germany.

Andersone, N.L. 1993. Candida / Megabacteria Proventikulitis in a Lesser Sulphur-Crested Cockatoo (Cacatua sulphure sulphurea). Journal of American Avian Veterinarian 1993 : Florida.

Bywater, R.J. 1991. The Control of Infectious Disease : Chemotherapy in Veterinary Apllied Pharmacology dan Therapeutics. Edited by G.C. Brader, D.M. Pugh, R.J. Bywater and W.L. Jenkins. 5th Edition. ELBS, Bailliere Tindal, London.

Carter, G.R.1979. Diagnostic Procedure in Veterinary Bacteriology and Micology.8th Edition. Charles C. Thomas Publisher, Illionis.

Hendrikz, J.K. and Philippich, L.J. 1998. Prevalence of Megabacteria in Budgerigar Colonies. School Veterinary Science and statistics Office. The University of Queensland. Queensland 4072.

Jang, S.S. and Biberstein, E.L. 1978. A Diagnostic Manual of Clinical Bacteriology and Micology. Unesco/Cida Regional Training Course in Veterinary Diagnostic Microbiology. Paradeniya, 4th-22nd August 1980.

Latimer, S.K., Wilson, G.H. and Son, T.T. 2002. Clinical and Pathological Features of Megabacteriosis in Bird. Departement of Pathology (Latimer). College of Veterinary Madice. The University of Georgia. Athens. GA 30602-7388.

Levine, M. 1954. An Introduction to Laboratory Technique In Bacteriology, Third Edition. Copyright 1954 by The Macmillan Company, Newyork.

Rahardja, K dan Tjay, T.H. 1991. Obat-obat Penting Khasiat, Penggunaan dan Efek-efek Sampingnya. Direktur Jendral Pengawasan Obat dan Makanan Departemen Kesehatan Republik Indonesia. Edisi kedua. Cetakan keempat. Jakarta.

Setiabudy, R., Sjamsudin, U., dan Bustami, Z.S. 1995. Famakologi dan Terapi, Bagian Farmakologi Fakultas Kedokteran Universitas Indonesia Jakarta, Edisi ketiga. PT Gaya Baru. Jakarta.

Talltree, C. 2000. Megabacteria a Review of The Literature. Journal of Association of Veterinarians-Australia. Email at Talltree@nwnet.net

Tennant, B. 2002. BSVA Small Animal Furmulary. 4th Edition. Copyright 2002 BSVA. Published by British Small Veterinary Association Woodrow House, Telford Way, Waterwells Bussiness Park, Quedgeley, Gloucester GL24B.

Werther, K.S.I., Verona C.E.S., and barros, L.S.S. 1997. The Occurence of an Megabacterium-like Organism at Small Bird from the Northeast area of Sao Paulo State brazil, Depto de Patologia Veterinaria / FCAV UNESP Via de Acesso Prof Paulo Donato Castellane s/n 14870-000-Jaboticabal-SP-Brazil.

Article Details

Author Biography

Miranti Candrarisna, Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Laboratorium Farmasi Veteriner Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya