PROTECTIVE EFFECTS OF COMBINED VITAMIN E AND ZINC SULFATE ON OVARIAN AND UTERUS WEIGHT OF ALBINO RATS (Rattus norvegicus) EXPOSED TO LEAD ACETATE
Article Sidebar
Main Article Content
Abstract
Lead exposure exerts toxic effects on the female reproductive system, particularly on the ovaries and uterus, resulting in hypofunction and organ atrophy. Oxidative stress induced by lead accumulation may be attenuated by antioxidants such as vitamin E and zinc sulfate. This study aimed to evaluate the protective effects of vitamin E, zinc sulfate, and their combination on ovarian and uterus weights in albino rats (Rattus norvegicus) exposed to lead acetate. Twenty-five female rats were randomly divided into five groups (K, T0, T1, T2, and T3) and treated orally for 21 days. On day 22, the rats were euthanized, and the ovaries and uterus were collected and weighed. The average ovarian weights for K, T0, T1, T2, and T3 were 25.80 ± 2.49; 18.62 ± 4.36; 28.40 ± 5.67; 33.78 ± 6.09; and 36.84 ± 5.15 mg, respectively. The average uterus weights for K, T0, T1, T2, and T3 were 292.48 ± 36.81; 249.96 ± 25.97; 315.86 ± 26.56; 325.54 ± 31.53; and 375.00 ± 36.55 mg, respectively. In conclusion, vitamin E and zinc sulfate demonstrated protective effects against lead-induced reproductive toxicity, with the combined treatment showing the greatest improvement in ovarian and uterus weights.
Article Details
Section
How to Cite
References
Amadi CN, Offor S.J., Frazzoil, C and Orisakwe, O.E., 2019. Natural Antidotes and Management of Metal Toxicity. Environmental Science and Pollution Research 1:1-21. https://doi.org/10.1007/s11356-019-05104-2
Ainun M, Hana E, Widjiati Wi, Wurlina W. 2021. Antioxidant α Tocopherol Against Leydig Cell Number, Testosterone and MDA Level and SOD activity in Rats Exposed to 2,3,7,8- terachlorodibenso-p-dioxin (TCDD). Vol 13. https://doi.org/10.31838/ijpr/2021.13.02.157
Beredugo, S., Ilegbedion, I. G., Tabowei, W., & Uchuno, G. A. 2022. Assessment of the Therapeutic Potentials of Selenium and Vitamin E on Ovarian and Renal Tissues of Lead Exposed Rats. International Journal of Recent Innovations in Medicine and Clinical Research (ISSN: 2582-1075), 4(2), 1-9. https://doi.org/10.18231/j.ijrimcr.2022.008
Berniyanti, T., Palupi, R., Kriswandini, I. L., Bramantoro, T., & Putri, I. L. 2020. Suitability of MDA, 8-OHdG and wild-type p53 as genotoxic biomarkers in metal (Co, Ni and Cr) exposed dental technicians: a cross-sectional study. BMC oral health, 20(1), 65. https://doi.org/10.1186/s12903-020-1049-1
BPOM RI. 2018. Peraturan Badan Pengawas Obat dan Makanan Nomor 5 Tahun 2018 Tentang Batas Maksimum Cemaran Logam Berat Dalam Pangan Olahan. Badan Pengawas Obat dan Makanan. Jakarta.
de Souza, I. D., de Andrade, A. S., & Dalmolin, R. J. S. 2018. Lead-interacting proteins and their implication in lead poisoning. Critical reviews in toxicology, 48(5), 375-386. https://doi.org/10.1080/10408444.2018.1429387
Di Meo, S., & Venditti, P. 2020. Evolution of the knowledge of free radicals and other oxidants. Oxidative medicine and cellular longevity, 2020(1), 9829176. https://doi.org/10.1155/2020/9829176
Garner, T.B., Hester, J.M., Carothers, A. and Diaz, F.J. 2021. Role of Zinc in Female Reproduction. Biology of Reproduction. 104(5): 976-989. https://doi.org/10.1093/biolre/ioab023
Li, C., Zhao, K., Zhang, H., Liu, L., Xiong, F., Wang, K., & Chen, B. 2018. Lead exposure reduces sperm quality and DNA integrity in mice. Environmental Toxicology, 33(5), 594-602. https://doi.org/10.1002/tox.22545
Mesalam, N. M., Ibrahim, M. A., Mousa, M. R., & Said, N. M. (2023). Selenium and vitamin E ameliorate lead acetate-induced hepatotoxicity in rats via suppression of oxidative stress, mRNA of heat shock proteins, and NF-kB production. Journal of Trace Elements in Medicine and Biology, 79, 127256. https://doi.org/10.1016/j.jtemb.2023.127256
Mumtaz, S., Ali, S., Khan, R., Shakir, H.A., Tahir, H.M., Mumtaz, S. and Andleeb, S. 2020. Therapeutic Role of Garlic and Vitamins C and E Against Toxicity Induced by Lead on Various Organs. Environmental Science and Pollution Research, 27(9), pp.8953-8964. https://doi.org/10.1007/s11356-020-07654-2
Ningtyas, N. S. I. I., Tirtasari, K., & Agustin, A. L. D. 2019. Proteksi Minyak Buah Merah (Pandanus Conoideus Lam.) Terhadap Jumlah Folikel Yang Terpapar Plumbum. Jurnal Sangkareang Mataram, 5(4), 7-9. https://sangkareang.org/index.php/SANGKAREANG/article/view/35
Nurkarimah, D. A., E. P. Hestianah, R. S. Wahjuni, M. Hariadi, S. Kuncorojakti. and H. A. Hermadi. 2017. Effect of Propolis on Spermatogenic Cells Number and Diameter of Seminiferus Tubules in Male Mice (Mus musculus). KnE Life Sciences. 3(6):677-683. https://doi.org/10.18502/kls.v3i6.1197
Raharjo, Y.Y.C.Y.A., Samsuri, I. and Berata, I.K. 2018. Gambaran Histopatologi Ovarium Tikus Putih (Rattus norvegicus) Akibat Pemberian Vitamin E dan Etinil. https://doi.org/10.19087/imv.2018.7.2.132
Sundari, D., Hananto, M. and Suharjo, S., 2016. Heavy Metal in Food Ingredients in Oil Refi nery Industrial Area, Dumai. Buletin Penelitian Sistem Kesehatan, 19(1), pp.55-61. https://doi.org/10.22435/hsr.v19i1.4989.55-61
Suksmerri., 2018. Dampak Pencemaran Logam Timah Hitam (Pb) terhadap Kesehatan. Jurnal Kesehatan Masyarakat Andalas, 2(2), pp.200-202. https://doi.org/10.24893/jkma.v2i2.34
Soemardini, S. and Prijadi, B., 2016. Pengaruh Pemberian Vitamin E Pada Tikus (Rattus norvegicus) Bunting yang Dipapar Asap Rokok Subakut terhadap Berat Badan Bayi Lahir Aterm. Majalah Kesehatan FKUB, 3(2), pp.76-85. https://doi.org/10.21776/ub.majalahkesehatan.003.02.4
Soussi, A., Gargouri, M., El Feki, A. 2018. Effects of co-exposure to lead and zinc on redox status, kidney variables, and histopathology in adult albino rats. Toxicol. Ind.Health 34: 469–480. https://journals.sagepub.com/doi/abs/10.1177/0748233718770293
Saeed, N.A.H.A.A, Al-Kadmy, I.M., Ibraheem, L.J., Al-Lami, H. F. D., and Aziz, S. N., 2017. The effect of Soybean (Glycine max) Aqueous Extract on Ovariann Structure in White Female Rats. Mintage Journal of Pharamaceutical and Medical Scinece, 21-22. DOI 10.20473/jmv.vol1.iss3.2018.120-127
Simanjuntak, E. J., dan Zulham, Z. 2020. Superoksida Dismutase (SOD) dan Radikal Bebas. Jurnal Keperawatan dan Fisioterapi (JKF), 2(2), 124-129. https://doi.org/10.35451/jkf.v2i2.342
Sari, A.I., Destriani, S.N., Soeharto, S. and Raharjo, B., 2018. Aktivitas Antioksidan Kitosan Pada Ovarium Tikus Betina yang Terpapar Timbal (Pb) Asetat. Fakultas Kedokteran Universitas Brawijaya. https://doi.org/10.31290/jiki.v(4)i(2)y(2018).page:80-86
Uchewa, O. O., & Ezugworie, O. J. 2019. Countering the effects of lead as an environmental toxicant on the microanatomy of female reproductive system of adult wistar rats using aqueous extract of Ficus vogelii. Journal of Trace Elements in Medicine and Biology, 52, 192-198. https://doi.org/10.1016/j.jtemb.2018.12.016
Wardani, D., Rahmanisa, S. and Puspita Sari, R.D. 2018. Pengaruh Kombinasi Tomat (Solanum lycopersicum L.) dan Zink Terhadap Jumlah Oosit Tikus Putih Betina Galur Sprague Dawley yang Diinduksi Gelombang Elektromagnetik Radiasi Ponsel. Majority, 7(3), pp.7-11. http://repository.lppm.unila.ac.id/10603/