UJI TOKSISITAS AKUT EKSTRAK DAUN SENDOK (Plantago major) PADA MENCIT(Mus musculus)
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Published:
2025-11-28
Keywords:
Acute, Harmless, Plantago major, Toxicity test
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Abstract
The leaves of Plantago major possess potential mostly for antioxidant, immunostimulant, and laxative properties. The study is to examine the impact of Plantago major leaves extract administration on 25 mice to ascertain the LD50 value in the acute toxicity assessment. Twenty-five female mice were allocated into five groups according to normal control group, negative control group and the dosage of Plantago major leaves extract, with doses of 10, 15, and 20 g/kg body weight. Observations were conducted over a 14-day period, encompassing characteristics such as body weight, physiological responses, clinical symptoms, absolute and relative organ weights, and daily mortality counts. Mortality and clinical signs were examined for 14 days after the treatment. The administration of Plantago major leaves extract to mice did not significantly affect body weight, not absolute or relative organ weight. The collected organs exhibited no severe lesions. The LD50 value of Plantago major leaves extract was determined to be more than 20 g/kg BW. It is concluded that LD50 value is considered as relatively harmless.
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UJI TOKSISITAS AKUT EKSTRAK DAUN SENDOK (Plantago major) PADA MENCIT(Mus musculus). JV [Internet]. 2025 Nov. 28 [cited 2025 Nov. 27];15(2):51-7. Available from: https://vitek-fkh.uwks.ac.id/jv/article/view/358
References
Addoun, N., Boual, Z., Delattre, C., Ursu, A.V., Desbrières, J., Le Cerf, D., Pierre, G. 2020. Structural features and rheological behavior of a water-soluble polysaccharide extracted from the seeds of Plantago ciliata Desf. International Journal of Biological Macromolecules, 155, 1333-1341.
Adom, M.B., Taher, M., Mutalabisin, M.F., Amri, M.S., Abdul Kudos, M.B., Wan Sulaiman, M.W.A., Sengupta, P., Susanti, D. 2017. Chemical constituents and medical benefits of Plantago major. Biomed Pharmacother., 96, 348-360.
Andriyanto, Aurellia, S., Putra, H.Y., Fadholly, A., Mustika, A.A., Pristihadi, D.N., Subangkit, M., Aryaliz, T. 2025. Evaluation of acute toxicity in mice administered with ashwagandha (Withania somnifera) suspension. Journal of Medicinal and Pharmaceutical Chemistry Research, 7: 2397-2410
Animal Care and Use Committee. 2009. Species Spesific Information: Mouse. Diakses pada 15 Mei 2025. Pukul 13.00 WIB. http://web.jhu.edu/ animalcare/ procedures/ mouse.html
Ayun, A.Q., Faridah, D.N., Yuliana, N.D., Andriyanto. 2021. Pengujian toksisitas akut LD50 infusa benalu teh (Scurrula sp.) dengan menggunakan mencit (Mus musculus). Acta Vet Indones., 9(1), 53-63.
Chinedu, E., Arome, D., Ameh, F.S. 2013. A new method for determining acute toxicity in animal models. Toxicol Int., 20(3), 224-6.
Elfahmi, Woerdenbag, H.J., Kayser, O. 2014. Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. Journal of Herbal Medicine, 4(2), 51–73.
Erhirhie, E.O., Ihekwereme, C.P., Ilodigwe, E.E. 2018 Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance. Interdiscip Toxicol., 11(1), 5-12.
Fadholly, A.F., Sadiah, S., Pristihadi, D.N., Harlina, E., Ramadhan, M.K., Budiharjo, Y.A.K., Adinugroho, C.R., Rahmah, A.A., Azizah, N.F. 2025. Unveiling the Safety of Cassia alata Leaf Extract: A Subchronic Oral Toxicity in Rats. Adv Anim Vet Sci., 13(4), 17-823.
Jegnie, M., Abula, T., Woldekidan, S., Chalchisa, D., Asmare, Z., Afework, M. 2023 Acute and sub-acute toxicity evaluation of the crude methanolic extract of Justicia schimperiana leaf in wistar albino rats. J Exp Pharmacol., 15, 467-483.
Jumiarni, W.O., Komalasari, O. 2017. Eksplorasi jenis dan pemanfaatan tumbuhan obat pada masyarakat suku wuna di pemukiman kota wuna. Traditional Medicine Journal, 22(1), 45-56.
Keshavarzi, A., Montaseri, H., Akrami, R., Moradi Sarvestani, H., Khosravi, F., Foolad, S., Zardosht, M., Zareie, S., Saharkhiz, M.J., Shahriarirad, R. 2022. Therapeutic efficacy of great plantain (Plantago major L.) in the treatment of second-degree burn wounds: a case-control study. Int J Clin Pract., 4923277.
Manekeng, H.T., Mbaveng, A.T., Ntyam Mendo, S.A., Agokeng, A.D., Kuete, V. 2019. Evaluation of acute and subacute toxicities of Psidium guajava methanolic bark extract: a botanical with in vitro antiproliferative potential. Evid Based Complement Alternat Med., 8306986.
Markov, D.D., Novosadova, E.V. 2022. Chronic unpredictable mild stress model of depression: possible sources of poor reproducibility and latent variables. Biology (Basel), 11(11), 1621.
Najafian, Y., Hamedi, S.S., Farshchi, M.K., Feyzabadi, Z. 2018. Plantago major in Traditional Persian Medicine and modern phytotherapy: a narrative review. Electron Physician, 10(2), 6390-6399.
[OECD423] The Organization for Economic Co-Operation and Development. 2001. OECD Guideline for Testing of Chemicals: Acute Oral Toxicity-Acute Toxic Class Method. USA: The Organization for Economic Co-Operation and Development.
Piao, Y., Liu, Y., Xie, X. 2013. Change trends of organ weight background data in sprague dawley rats at different ages. J. Toxicol. Pathol., 2, 29–43.
Rasekh, H,R,, Hosseinzadeh, L., Mehri, S., Kamli-Nejad, M., Aslani, M., Tanbakoosazan, F. 2012. Safety assessment of Ocimum basilicum hydroalcoholic extract in wistar rats: acute and subchronic toxicity studies. Iran. J. Basic Med. Sci., 15(1), 645–653.
Sulastra, C.S., Khaerati, K., Ihwan. 2020. Toksisitas akut dan lethal dose (LD50) ekstrak etanol uwi banggai ungu (Dioscorea alata L.) pada tikus putih (Rattus novergicus). Jurnal Ilmiah Medicamento, 6(1), 10-14.
Zhang, S., Hu, J., Sun, Y., Huizi, T., Yin, J., Geng, F., Nie, S. 2021. Review of structure and bioactivity of the Plantago (Plantaginaceae) polysaccharides. Food Chemistry, 12, 1-9.
Adom, M.B., Taher, M., Mutalabisin, M.F., Amri, M.S., Abdul Kudos, M.B., Wan Sulaiman, M.W.A., Sengupta, P., Susanti, D. 2017. Chemical constituents and medical benefits of Plantago major. Biomed Pharmacother., 96, 348-360.
Andriyanto, Aurellia, S., Putra, H.Y., Fadholly, A., Mustika, A.A., Pristihadi, D.N., Subangkit, M., Aryaliz, T. 2025. Evaluation of acute toxicity in mice administered with ashwagandha (Withania somnifera) suspension. Journal of Medicinal and Pharmaceutical Chemistry Research, 7: 2397-2410
Animal Care and Use Committee. 2009. Species Spesific Information: Mouse. Diakses pada 15 Mei 2025. Pukul 13.00 WIB. http://web.jhu.edu/ animalcare/ procedures/ mouse.html
Ayun, A.Q., Faridah, D.N., Yuliana, N.D., Andriyanto. 2021. Pengujian toksisitas akut LD50 infusa benalu teh (Scurrula sp.) dengan menggunakan mencit (Mus musculus). Acta Vet Indones., 9(1), 53-63.
Chinedu, E., Arome, D., Ameh, F.S. 2013. A new method for determining acute toxicity in animal models. Toxicol Int., 20(3), 224-6.
Elfahmi, Woerdenbag, H.J., Kayser, O. 2014. Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. Journal of Herbal Medicine, 4(2), 51–73.
Erhirhie, E.O., Ihekwereme, C.P., Ilodigwe, E.E. 2018 Advances in acute toxicity testing: strengths, weaknesses and regulatory acceptance. Interdiscip Toxicol., 11(1), 5-12.
Fadholly, A.F., Sadiah, S., Pristihadi, D.N., Harlina, E., Ramadhan, M.K., Budiharjo, Y.A.K., Adinugroho, C.R., Rahmah, A.A., Azizah, N.F. 2025. Unveiling the Safety of Cassia alata Leaf Extract: A Subchronic Oral Toxicity in Rats. Adv Anim Vet Sci., 13(4), 17-823.
Jegnie, M., Abula, T., Woldekidan, S., Chalchisa, D., Asmare, Z., Afework, M. 2023 Acute and sub-acute toxicity evaluation of the crude methanolic extract of Justicia schimperiana leaf in wistar albino rats. J Exp Pharmacol., 15, 467-483.
Jumiarni, W.O., Komalasari, O. 2017. Eksplorasi jenis dan pemanfaatan tumbuhan obat pada masyarakat suku wuna di pemukiman kota wuna. Traditional Medicine Journal, 22(1), 45-56.
Keshavarzi, A., Montaseri, H., Akrami, R., Moradi Sarvestani, H., Khosravi, F., Foolad, S., Zardosht, M., Zareie, S., Saharkhiz, M.J., Shahriarirad, R. 2022. Therapeutic efficacy of great plantain (Plantago major L.) in the treatment of second-degree burn wounds: a case-control study. Int J Clin Pract., 4923277.
Manekeng, H.T., Mbaveng, A.T., Ntyam Mendo, S.A., Agokeng, A.D., Kuete, V. 2019. Evaluation of acute and subacute toxicities of Psidium guajava methanolic bark extract: a botanical with in vitro antiproliferative potential. Evid Based Complement Alternat Med., 8306986.
Markov, D.D., Novosadova, E.V. 2022. Chronic unpredictable mild stress model of depression: possible sources of poor reproducibility and latent variables. Biology (Basel), 11(11), 1621.
Najafian, Y., Hamedi, S.S., Farshchi, M.K., Feyzabadi, Z. 2018. Plantago major in Traditional Persian Medicine and modern phytotherapy: a narrative review. Electron Physician, 10(2), 6390-6399.
[OECD423] The Organization for Economic Co-Operation and Development. 2001. OECD Guideline for Testing of Chemicals: Acute Oral Toxicity-Acute Toxic Class Method. USA: The Organization for Economic Co-Operation and Development.
Piao, Y., Liu, Y., Xie, X. 2013. Change trends of organ weight background data in sprague dawley rats at different ages. J. Toxicol. Pathol., 2, 29–43.
Rasekh, H,R,, Hosseinzadeh, L., Mehri, S., Kamli-Nejad, M., Aslani, M., Tanbakoosazan, F. 2012. Safety assessment of Ocimum basilicum hydroalcoholic extract in wistar rats: acute and subchronic toxicity studies. Iran. J. Basic Med. Sci., 15(1), 645–653.
Sulastra, C.S., Khaerati, K., Ihwan. 2020. Toksisitas akut dan lethal dose (LD50) ekstrak etanol uwi banggai ungu (Dioscorea alata L.) pada tikus putih (Rattus novergicus). Jurnal Ilmiah Medicamento, 6(1), 10-14.
Zhang, S., Hu, J., Sun, Y., Huizi, T., Yin, J., Geng, F., Nie, S. 2021. Review of structure and bioactivity of the Plantago (Plantaginaceae) polysaccharides. Food Chemistry, 12, 1-9.